Journal:

Article Title: Interleukin-6 Enhances Production of Anti-OspC Immunoglobulin G2b Borreliacidal Antibody

doi: 10.1128/IAI.69.7.4268-4275.2001

Figure Lengend Snippet: Titers of borreliacidal antibody in supernatants obtained from cultures of lymph node cells containing B. burgdorferi isolate 50772 and 1, 10, or 100 μg of anti-IL-6 after incubation for 7 days. Lymph node cells were obtained from C3H/HeJ mice 28 days after vaccination. Control cultures were treated with a goat isotype-nonspecific IgG antibody.

Article Snippet: They were then washed five times with PBS-T and incubated for 45 min at 21°C with 50 μl of biotinylated rat anti-murine IL-6 (MP5-32C11 clone; PharMingen) diluted in PBS-FBS to 1 μg/ml.

Techniques: Incubation

Journal:

Article Title: Interleukin-6 Enhances Production of Anti-OspC Immunoglobulin G2b Borreliacidal Antibody

doi: 10.1128/IAI.69.7.4268-4275.2001

Figure Lengend Snippet: Immunoblots of B. burgdorferi isolate 50772 after treatment with supernatant obtained from cultures of lymph node cells containing B. burgdorferi isolate 50772 and 100 μg of anti-murine IL-6. Supernatant was collected on day 7 of incubation. Lymph node cells were obtained from C3H/HeJ mice 28 or 35 days after vaccination. Control cultures were treated with a goat isotype-nonspecific IgG antibody.

Article Snippet: They were then washed five times with PBS-T and incubated for 45 min at 21°C with 50 μl of biotinylated rat anti-murine IL-6 (MP5-32C11 clone; PharMingen) diluted in PBS-FBS to 1 μg/ml.

Techniques: Western Blot, Incubation

Journal: BMC Cancer

Article Title: IL-6 signaling by STAT3 participates in the change from hyperplasia to neoplasia in NRP-152 and NRP-154 rat prostatic epithelial cells

doi: 10.1186/1471-2407-1-19

Figure Lengend Snippet: IL-6 expression of clones A4 (red) and D8 (green) of NRP-154 cells. Clones of NRP-154 cells were derived by limit dilution at 1 cell/well; calculated cloning efficiency was 17.8%. Clones were fixed, permeabilized, and stained with biotinylated anti-IL-6 (R&D Systems) plus streptavidin-phycoerythrin (Pharmingen), as described in Materials & Methods. Fluorescence in FL2 was analyzed on a FACScan. The IL-6 expression of the 16 clones was either high, like A4 (shown in red), or low, like D8 (shown in green). A4 cells are 8 times as fluorescent as D8 cells. The IL-6 expression of 2 of the 16 clones is shown for the sake of clarity. The fluorescence intensity of clone A4 was 10× that of clone D8 in this experiment (p < 0.001 by KS statistics). A histogram from 1 determination is shown here.

Article Snippet: Cells were blocked by incubation on ice in goat Ig (Sigma), 2 mg/ml, for 45 min. After washing twice, cells were incubated with 1 or 2 μg/10 6 cells in 100 μl biotinylated goat anti-human IL-6 receptor (ligand-affinity purified; R&D Systems) on ice for 45 min. After washing three times, cells were incubated with phycoerythrin-labeled streptavidin for 45 min on ice.

Techniques: Expressing, Clone Assay, Derivative Assay, Cloning, Staining, Fluorescence

Journal: Hypertension

Article Title: Downregulation of Renal TRPM7 and Increased Inflammation and Fibrosis in Aldosterone-Infused Mice

doi: 10.1161/hypertensionaha.107.100339

Figure Lengend Snippet: Figure 2. Immunohistochemical images demonstrate renal IL-6 content in the 4 groups. IL-6 is demonstrated as brown staining. IL-6 content was increased in the aldosterone group vs other groups. Magnesium reduced IL-6 content in aldosterone-treated mice. Images are representative of 8 to 9 mice in each group. Insert is a representative image from another section.

Article Snippet: For direct immunohistochemistry, sections were incubated with 3 % H2O2 and a Pierce solution to block endogenous peroxidase and biotin, respectively, followed by overnight incubation (humidified box, 4°C) with a biotinated anti-goat interleukin-6 (IL-6) monoclonal antibody (Santa Cruz).

Techniques: Immunohistochemical staining, Staining